P-75: Effects of Cholesterol Loaded Cyclodextrin on Cat Sperm Vitrification

Background: Cholesterol loaded cyclodextrin (CLC) efficiently protected sperm from some species. On the other hand vitrification of sperm especially in human opened a new horizon in the field of sperm freezing. The aim of the present study was to evaluate CLC on vitrification of cat sperm. Materials and Methods: Tom epididymal sperm (n=9) was extracted from epididymides and subjected to vitrification in TRIS base solution that was incorporated with CLC. The warmed sperm was analysed for motility and viability. Results: Vitrification significantly (p<0.0001) reduced the sperm motility (%) in CLC-plus (1.14 ± 0.02) and CLC-minus (4.2 ± 0.02) compared to fresh samples (25 ± 0.02). Sperm viability was also decreased significantly following vitrification either with CLC (80.3 ± 0.03; p=0.0063) or without CLC (79.3 ± 0.03; p=0.0159) compared to the fresh samples (p=0.7933). Conclusion: Cat sperm showed an indigenous tolerance to the vitrification procedure and CLC does not have any beneficial effect on it.